英语翻译We consistently observed higher expression of the 2XMBP–

来源：学生作业帮编辑：拍题作业网作业帮分类：英语作业时间：2024/04/29 21:42:47

英语翻译
We consistently observed higher expression of the 2XMBP–BRCA2 protein compared to MBP–BRCA2 both in stable VC8 clones,as well as in transient transfections of both VC8 and human 293T cells.Clones positive for expression were tested for complementation by clonogenic survival response to mitomycin C.

我们持续观察到不论在VC8细胞克隆群当中还是短暂转染的VC8和人体293t细胞,2XMBP–BRCA2 总比MBP–BRCA2 要更高效的表达.为了完善克隆基因对mitomycin C生存反应的测试,因而做了克隆阳性表达测验.
再问：请问这个呢 TotalRNA(1 mg) was used in each RT–PCR (Titanium One-step RT–PCR, Clontech) reaction containing either MBP primer set (RJ-59MBP2XN/RJ-39MBP2XN) to amplify the MBP tag (1.1 kb) or C-terminal primer set (RJ-598269/RJ-39AGEIBRCA2) to amplify the last 0.9 kb of the BRCA2 open reading frame.

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