英语翻译Different plant transformation constructs that were deri

英语翻译
Different plant transformation constructs that were derivatives of base vector pTF101.1 were introduced into Agrobacterium tumefaciens strain EHA101 (Hood et al.1986).The base vector pTF101.1 is a derivative of the pPZP binary vector (Hajdukiewicz et al.,1994) that includes the right and left T-DNA border fragments from a nopaline strain of A.tumefaciens,a broad host origin of replication (pVS1) and a spectinomycin-resistant marker gene (aadA) for bacterial selection.The plant selectable marker gene cassette
consists of (1) double 35S promoter (2x P35S) of cauliflower mosaic virus (CaMV) (Odell et al.1985),(2) tobacco etch virus translational enhancer (Carrington and Freed 1990),(3) the phosphinothricin acetyl transferase (bar) gene from Streptomyces
hygroscopicus that confers resistance to the herbicide phosphinothricin and its derivatives.).pTF101.1 contains a multiple cloning site (MCS) for facilitating subcloning of the gene of interest in between the right border region and the plant
selectable marker cassette.The soybean vegetative storage protein terminator (Mason et al.,1993) was cloned to the 3’ end of the bar gene.The vector pTF102 was derived from pTF101.1 by inserting the P35S GUS intron cassette (Vancanneyt et al.1990) into the
Hind III site of pTF101.1 The gus gene contained a portable intron in its codon region (Vancanneyt et al.,1990) to prevent GUS activity in Agrobacterium cells.
呵呵，可是机器翻的我看不懂啊

不同的植物转化质粒被衍生物基地载体ptf101.1引入农杆菌株植(罩】 .1986年) .基地ptf101.1载体是一个派生的ppzp二进制向量( hajdukiewicz 】 .,1994 ) ,包括左,右笔-脱氧核糖核酸片段边界从胭脂株农杆菌,广阔的主持人出身的复制( pvs1 )和大观霉素抗性标记基因( aada所) ,为细菌的选择.植物标记基因的暗盒
共分( 1 )双35s启动模式( 2x p35s )花椰菜花叶病毒(与camv ) ( odell 】 .1985年) ,( 2 )烟草蚀纹病毒平移强化剂(卡林顿并释放1990 ) ,( 3 )膦乙酰转移酶(酒吧)的基因链霉菌
吸水赋予抗除草剂膦及其衍生物) ..ptf101.1含有多种克隆网站(成员国)为便利亚克隆基因兴趣权之间的边境地区和电厂
选择标记盒.大豆营养贮藏蛋白终结者(梅森】 .,1993 )中克隆到3 '端的bar基因.矢量ptf102来自ptf101.1插入p35s gus基因内含盒( vancanneyt 】 .1990 )进入
三后腿部位ptf101.1的gus基因包含一个便携式内含其密码区域( vancanneyt 】 .,1990 ) ,以防止gus活性农杆菌细胞.