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英语翻译At low magnification,single cells grew as a homogeneous

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英语翻译
At low magnification,single cells grew as a homogeneous monolayer of cells with cobblestone-like appearance.The cells were structurally polarized:the apical pole,facing the free surface,differed from the basal pole,toward the underlying support layer.The presence of specific markers,like transporter proteins or brush border enzymes,was established to characterize these primary cultures.The PTC shows a clear immunoreactivity against AQP1,while no positive signal for early distal tubule expressing AQP2 or NCC was obtained.The multiligand receptor,megalin,is located at the brush border of PT cells and participates in protein reabsorption from the primitive urine.The chloride channel,ClC-5,and the vacuolar proton pump,V-ATPase,are both localized in endosomes ensuring correct endosomal acidification and protein trafficking in PT cells.All of these components of the endocytic apparatus were detected in primary PT cells
低倍镜下,成长为一个单个细胞的细胞与cobblestone-like单层均匀.这些细胞是结构性的顶端极极化,面向自由面,有别于基底杆,向潜在支持层.在场的情况下,如转运蛋白的特殊标记或刷边境酶,建立了描述这些主要的文化.PTC显示immunoreactivity攻击,而不积极的信号AQP1表达AQP2远为早期发生.应或者这个multiligand受体,megalin,位于边境的笔刷和参与PT细胞蛋白质增高从原始的尿液.氯离子通道,ClC-5、金属质子泵、V-ATPase,都是确保正确定位endosomes内酸化和蛋白质贩卖人口的细胞.PT所有这些部件的endocytic仪进行检测的主要PT细胞