英语翻译The strain Spirulina platensis UTEX 1926,recently re-cla

英语翻译
The strain Spirulina platensis UTEX 1926,recently re-classified as Arthrospira (Spirulina) platensis (Nordstedt) Gomont ,was obtained from the University of The strain Spirulina platensis UTEX 1926,recently re-classified as Arthrospira (Spirulina) platensis (Nordstedt) Gomont ,was obtained from the University of Texas Culture Collection.Two different types of media were used.The medium of Schlösser
with NaNO3 as a nitrogen source was utilized for microorganism maintenance and inoculum preparation,whereas a modified medium was utilized for cultivations,where NaNO3 was substituted by urea as nitrogen source.The total fed mass of urea was a function of the total feeding time.Cells for the inoculum were
grown in 500mL Erlenmeyer flasks containing 200mL of medium,kept on rotary shaker at 100min−1,temperature of 30 ◦C and light intensity of 72\2mol photonsm−2 s−1 [25].After achievementof the exponential phase,they were harvested and used to seed elongated minitanks (light-exposed area of 0.123m2) made of PVC laminas simulating large-scale outdoor ponds .Cultivations were carried out at a paddle wheel rotation of 18 min−1,28◦C and a constant working volume of 5.0 L ensured by daily addition of distilled water to replace its evaporation.The continuous light intensity was regulated at 108\2mol photonsm−2 s−1 [23] by means of six 20W fluorescent lamps and an illuminance meter TL-1 (Minolta,Osaka,Japan).Light intensity was initially expressed in klx,and then converted to photosynthetic photon flux density (PPFD),expressed in \2mol photons m−2 s−1,using the conversion
factor proposed by McCree for white fluorescent light (12\2mol photonsm−2 s−1 klx−1).An initial biomass concentration of 50mgL−1 (dry weight) was always used at the start of each fed-batch culture [29] that preceded three successive repeated fed-batch cycles.In order to avoid growth inhibition due to excess of ammonia,the initial nitrogen concentration was set at 2.67mM,and the feeding time of the preliminary
fed-batch phase was set in 14 days .According to the experimental protocol listed in Table 1,in all the repeated fed-batch cultivations,aliquots of a concentrated urea solution were daily added to the medium to give a nitrogen concentration of 1.50mM
.For this purpose,the following equation was used to ensure a constant feeding rate:N = a + bt (1) where N is the nitrogen concentration added up to a time t,a = 2.67mM that present at the beginning of the cultivation and of each cycle,and b = 1.5mMd−1 is a parameter which the fedbatch feeding pattern depends on.According to the ratio between renewed and total volumes (R),the total feeding time (tf) varied from1 to 16 days.
复制粘贴原因,有些符号不准确.段中“mol”前都有个 μ ,m2是指㎡,以此类推m-2等 .主要是专业术语,好的话我会多加30分.

德克萨斯大学菌种中心通过将钝顶螺旋藻（UTEX 1926）从组的方式得到Arthrospira (Spirulina) platensis (Nordstedt) Gomont.这里使用了两种培养液.一种是含有NaNO3的Schlösser培养液,它能提供氮源用以保存微生物并预备种菌.相反,第二种培养液把NaNO3替换为尿素,用作培养细菌的氮源.尿素总供给量随总供给时间而变化.在500mL锥形烧瓶内装有200mL的培养液来培养种菌,并被置于速度为100min−1的旋转振荡器上,温度为30 ◦C,光度为72\2mol photons m−2 s−1 [25].完成对数生长期后,它们被取出置于用PVC薄板制成的细长型的容器内（这种容器光照面积为0.123m2,可模拟大型室外池塘的环境）.具体培养环境为：18 min−1的叶轮搅拌、28◦C、5.0 L的恒定培养容积（保证措施：每天加注蒸馏水补偿蒸发量）.通过六盏20W的荧光灯和TL-1 (Minolta, Osaka, Japan)型照度表来恒定108\2mol photonsm−2 s−1 [23]的光强度.光强度初始被?,然后被调整到光合光量子通量密度,用McCree提出的白色荧光灯的换算因数(12\2mol photonsm−2 s−1 klx−1),照射光度为 \2mol photons m−2 s−1.在三个连续分级喂料周期前,每批次培养的初始生物量浓度被控制在50mgL−1 (干重).为了避免过量的氨抑制生长,初始氮浓度被设定为2.67mM,初始分批喂料期被设定为14天.根据表1的实验数据,每天添加氮浓度为1.50mM的培养液.参照以下公式以保持一致的喂料率：N = a + bt (1) 其中N表示前后相加的总氮浓度. a = 2.67mM 表示开始的中容积,总喂料时间从1天到16天不等.
时间急 来不及理解该文章 暂时这样吧 需要的话再修改