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设计一个实验进行特定基因的克隆测序

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设计一个实验进行特定基因的克隆测序
If you are trying to clone a known gene,it will be easy.You can clone it either by directly cloning a restriction fragment or by PCR the gene and clone it.After that,you can sequence it to make sure you do not introduce any mutations into the clone.
It depends on what you want,if you just want gene,then you have to begin with chromosomal DNA.If you like to clone cDNA,then you have to begin with RNA.
Good luck!