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生物信息中的reads contig scaffold N50 具体点

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生物信息中的reads contig scaffold N50 具体点
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Contigs and scaffolds
Contig The result of joining an overlapping collection of sequences or clones.
Scaffold The result of connecting contigs by linking information from paired-end reads from plasmids,paired-end reads from BACs,known messenger RNAs or other sources.The contigs in a scaffold are ordered and oriented with respect to one another.
Fingerprint clone contigs Contigs produced by joining clones inferred to overlap on the basis of their restriction digest fingerprints.
Sequenced-clone layout Assignment of sequenced clones to the physical map of fingerprint clone contigs.
Initial sequence contigs Contigs produced by merging overlapping sequence reads obtained from a single clone,in a process called sequence assembly.
Merged sequence contigs Contigs produced by taking the initial sequence contigs contained in overlapping clones and merging those found to overlap.These are also referred to simply as 'sequence contigs' where no confusion will result.
Sequence-contig scaffolds Scaffolds produced by connecting sequence contigs on the basis of linking information.
Sequenced-clone contigs Contigs produced by merging overlapping sequenced clones.
Sequenced-clone-contig scaffolds Scaffolds produced by joining sequenced-clone contigs on the basis of linking information.
Draft genome sequence The sequence produced by combining the information from the individual sequenced clones (by creating merged sequence contigs and then employing linking information to create scaffolds) and positioning the sequence along the physical map of the chromosomes.
N50 length A measure of the contig length (or scaffold length) containing a 'typical' nucleotide.Specifically,it is the maximum length L such that 50% of all nucleotides lie in contigs (or scaffolds) of size at least L.