英语翻译The plasmid pRL-null,which encodes Renilla luciferase,wa

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英语翻译
The plasmid pRL-null,which encodes Renilla luciferase,was purchased from Promega.Plasmids expressing a dominant-active (da)-Ras (pSV/H-RasV12) and dominant-negative (dn)-Ras (pSV/H- RasN17) were kindly provided by Dr.P.-G.Suh (Department of Life Science,Pohang University of Science and Technology,Korea).The plasmids for dn-Raf1 (pSV/Raf1 DN),dn-MEK1 (pCGN1/MEK DN),dn- Erk2 (pHA-Erk2 K52R),dn-JNK1 (pSRα/HA-JNK T183A/Y185F),and dn-p38 kinase (pCDNA3/flag-p38 T180A/Y182F) were kindly provided by Dr.D.S.Min (Department of Molecular Biology,College of Natural Science,Pusan National University,Korea).dn-Egr-1 (pCDNA3.1/ Egr1N372) was generated as previously described (Gosslar et al.,1999).

该质粒pRL - null,它编码海肾荧光素酶,购自试剂盒.质粒表达主导积极的（大）,拉斯（pSV/H-RasV12）和显性负（DN）的-拉斯（pSV/H- RasN17）热心提供了博士P.-G.瑞（生命科学系,浦项科技,高丽大学）.为DN的质粒,Raf1（pSV/Raf1的DN）的DN -激酶1（pCGN1/MEK的DN）,肾病,Erk2素（PHA - Erk2 K52R）,的DN - JNK1的（pSRα/医管局化JNK T183A/Y185F）和DN - p38激酶（pCDNA3/flag-p38 T180A/Y182F）热心提供了局副局长闵博士（分子生物学,自然科学,釜山国立大学部大学,韩国）.的DN - Egr - 1的（pCDNA3.1 / Egr1N372）生成如前所述（Gosslar等.,1999）.

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