作业帮帮忙解释下各基因片段的关系
来源：学生作业帮 编辑：拍题作业网作业帮 分类：英语作业 时间：2024/04/28 21:33:03
帮忙解释下各基因片段的关系
Cosmid vector pOJ446 and pSET152 were used in the construction of the genomic library and recombinant plasmid pSLT,respectively .
The recombinants pGEM-LT and pSLT were constructed by subcloning the pGEM-3zf and pSET152 integration vectors.
The amplified products (speA,speB and spcS2) were cloned into the XbaI and BamHI-digested pGEM-3zf subcloning vector to construct the pGEM-LT plasmid.
The integration plasmid pSLT was then constructed into the XbaI and BamHI-digested pSET152 integration vector,and the constructed recombinant containing speA,speB and spcS2 was verified by PCR.
The plasmid pSLT was transformed into the E.coli ET12567 host for demethylation.
The recombinant plasmid pSLT was constructed by inserting the speA,speB and spcS2 into integration vector pSET152 containing the ermE* promoter with ribosomebinding sites.
pOJ446,pSET152 ,pSLT,pGEM-LT,speA,speB,spcS2,pGEM-3zf,E.coli ET12567 各自关系 大概看明白了 可不确定
Cosmid vector pOJ446 and pSET152 were used in the construction of the genomic library and recombinant plasmid pSLT,respectively .
The recombinants pGEM-LT and pSLT were constructed by subcloning the pGEM-3zf and pSET152 integration vectors.
The amplified products (speA,speB and spcS2) were cloned into the XbaI and BamHI-digested pGEM-3zf subcloning vector to construct the pGEM-LT plasmid.
The integration plasmid pSLT was then constructed into the XbaI and BamHI-digested pSET152 integration vector,and the constructed recombinant containing speA,speB and spcS2 was verified by PCR.
The plasmid pSLT was transformed into the E.coli ET12567 host for demethylation.
The recombinant plasmid pSLT was constructed by inserting the speA,speB and spcS2 into integration vector pSET152 containing the ermE* promoter with ribosomebinding sites.
pOJ446,pSET152 ,pSLT,pGEM-LT,speA,speB,spcS2,pGEM-3zf,E.coli ET12567 各自关系 大概看明白了 可不确定
供参考：
pGEM-3zf质粒用Xbal和BamHI酶切后,连入speA、speB、spcS2片段构建pGEM-LT质粒.
pSET152质粒用Xbal和BamHI酶切后,连入speA、speB、spcS2片段构建pSLT质粒.
E.coli ET12567是菌株,克隆载体用的.
pOJ446没写,可能是用来提供speA、speB、spcS2基因片段的.
pGEM-3zf质粒用Xbal和BamHI酶切后,连入speA、speB、spcS2片段构建pGEM-LT质粒.
pSET152质粒用Xbal和BamHI酶切后,连入speA、speB、spcS2片段构建pSLT质粒.
E.coli ET12567是菌株,克隆载体用的.
pOJ446没写,可能是用来提供speA、speB、spcS2基因片段的.