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英语翻译基因治疗的主要途径有两条,即ex vivo与in vivo方式.in vivo途径需要载体将外源基因直接导入体内

来源:学生作业帮 编辑:拍题作业网作业帮 分类:英语作业 时间:2024/03/29 01:47:18
英语翻译
基因治疗的主要途径有两条,即ex vivo与in vivo方式.in vivo途径需要载体将外源基因直接导入体内.这种载体可以是病毒型或非病毒型.常用病毒载体有反转录病毒、腺病毒等.反转录病毒是通过与宿主细胞表面的特异受体相互作用而感染宿主细胞的.之后进入宿主细胞的胞浆中,用病毒颗粒中含有的反转录酶开始反转录病毒基因组的RNA,合成病毒基因组的线性DNA拷贝后,病毒DNA整合到宿主基因组中.反转录病毒载体需的病毒基因组成分、外源基因和标记基因.介导基因转移的一般过程大致包含六步:克隆目的基因,导入包装细胞,筛选转化细胞,选择培养,感染细胞,分析基因表达及治疗效果.
The gene treatment main way has two,namely ex vivo and in vivo way.In the vivo way needs the carrier directly to induct the extraneoussource gene in vivo.This kind of carrier may be the virus or the non-virus.The commonly used viral carrier has the reverse to record thevirus,the adenovirus and so on.The reverse records the virus isthrough mutually affects with the host cell surface special acceptorinfects the host cell.Enters the host cell afterwards 胞浆 center,includes the reverse with the viral pellet in to record the enzyme tostart to reverse records the viral gene group RNA,after the synthesisvirus gene group's linear DNA copy,viral DNA conformity to host genegroup in.The reverse records the viral gene group ingredient,theextraneous source gene and the mark gene which the viral carrierneeds.Lies between leads the gene shift the general processapproximately to contain six step:The clone goal gene,inducts thepacking cell,the screening transformation cell,the elective culture,the infection cell,the analysis gene expression and the treatmentresult.